Bio-Rad Experion Protein Analysis Kits - Manual (Page 21)

10. The data appear in the spreadsheet. Click on cell H1 (Peak Number) and select
Data > Sort to sort the data by peak number.
11. Insert 3 columns between columns I and J, and label them Mean Conc, Stdev, and
%CV, respectively. Use Excel software to determine the mean, standard deviation, and
%CV for samples 1–12 (Figure 1.11). The %CV for Test 1 using the mouse liver total
RNA sample provided is typically £15%
Fig. 1.11. Excel spreadsheet showing example of calculation of mean, standard deviation, and
%CV.
Expected Performance of Test 1 3 . Based on results obtained using 72 samples at 100 ng/µl.
Measurement Typical Results
Quantitation reproducibility %CV £ 15% for 100 ng/µl total RNA
Quantitation of RNA samples with the Experion system offers reproducibility and accuracy that
are comparable to other traditional methods, such as UV spectroscopy. It also offers a level of
sensitivity that is similar to that offered by fluorescent probes, such as the RiboGreen reagent
(Woo and Strong 2006)
3 .
If your results do not appear as they do in these examples, refer to the Troubleshooting section
of the Experion RNA analysis kit and Experion system instruction manuals or contact Bio-Rad
technical support. Typically, problems with reproducibility are due to problems with pipetting or
chip loading. For tips on optimizing your results, refer to the Experion Training Video: Chip
Loading and the Essential Practices and Troubleshooting sections of the Experion system
instruction manual.
3 For more detailed analyses of the expected performance of the Experion RNA StdSens assay, refer to the following
Bio-Rad tech notes:
Urban et al., Performance comparison of the Experion automated electrophoresis system and a competing automated
system for RNA analysis, Bio-Rad bulletin 5286 (2005)
Woo L and Strong W, RNA Quantitation: A comparative analysis using the Experion automated electrophoresis system,
RiboGreen reagent, and ultraviolet spectroscopy, Bio-Rad bulletin 5420 (2006)
17
10010511A.qxp 2/28/2008 7:14 AM Page 17