Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 79)

Caution: Use caution when handling radioisotopes. Proper handling and disposal of
radioactive material should be followed.
5. After the gel has been treated with a fluorographic reagent, carefully place the gel on a
3MM Whatman paper and remove any air bubbles. Place Saran Wrap evenly on top of the
gel without creating any bubbles. This helps to keep the gel intact and prevents any
contamination to the gel dryer. Place the gel in a gel dryer for about 60 minutes at 60°C.
6. Expose the gel to X-ray film or a phosphor imaging screen. Scan the imaging screen on
a storage phosphor imaging system.
Section 9
Troubleshooting
Always confirm that the line voltage is correct for the DCode system.
9.1 Equipment
Problem Cause Solution
Controller
No display with power on Burned out fuse Replace fuse located
near power cord
connection
Buffer not circulating Buffer level too low Add buffer to ‘Fill’
level
Pump clogged, not Call Bio-Rad
working
Cannot preheat buffer Buffer level too low Add buffer to ‘Fill’ level
Long preheat time Clear loading lid not Place clear loading lid on
on system system
Stir bar not functioning Broken belt on stirrer Replace belt
Stir bar interferes with Maximum thickness
gel sandwiches of gel is 1.5 mm
Stir bar not engaged Align stir bar in support
tank hole
Casting gels
Leaking during gel casting Improper assembly of Using the alignment
gel sandwich card, check that the
spacers and glass plate
bottom are flush prior to
pouring gel
Chipped glass plates Insure glass plates are
free of flaws. Use new set
of glass plates
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