Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 3)

Table of Contents
Section 1 General Safety Information ..........................................................1
1.1 Caution Symbols........................................................................................1
1.2 Precautions During Set-up .........................................................................1
1.3 Precautions During a Run ..........................................................................1
1.4 Precautions After a Run.............................................................................2
1.5 Safety ........................................................................................................2
Section 2 Introduction ...................................................................................2
2.1 Introduction to Mutation Detection Technology...........................................2
Section 3 Product Description ......................................................................2
3.1 Packing List ...............................................................................................2
3.2 System Components and Accessories.......................................................5
Section 4 Denaturing Gel Electrophoresis (DGGE, CDGE, TTGE)............11
4.1 Introduction to Denaturing Gradient Gel Electrophoresis (DGGE) ............11
Reagent Preparation................................................................................13
Gel Volumes ............................................................................................15
Sample Preparation .................................................................................16
Temperature Controller............................................................................17
Preheating the Running Buffer .................................................................18
Assembling the Perpendicular Gradient Gel Sandwich ............................18
Casting Perpendicular Gradient Gels .......................................................22
Assembling the Parallel Gradient Gel Sandwich ......................................24
Casting Parallel Gradient Gels .................................................................26
4.2 Introduction to Constant Denaturing Gel Electrophoresis (CDGE)............28
Reagent Preparation................................................................................29
Gel Volumes ............................................................................................31
Sample Preparation .................................................................................31
Temperature Controller............................................................................32
Preheating the Running Buffer .................................................................33
Assembling the CDGE Gel Sandwich ......................................................33
Casting CDGE Gels.................................................................................35
4.3 Introduction to Temporal Temperature Gradient Gel Electrophoresis
(TTGE) ....................................................................................................36
Calculating the Run Parameters ..............................................................37
Reagent Preparation................................................................................38
Gel Volumes ............................................................................................40
Sample Preparation .................................................................................40
Preheating the Running Buffer .................................................................40
Assembling the TTGE Gel Sandwich .......................................................40
Temperature Controller............................................................................41
Casting TTGE Gels..................................................................................43
Section 5 Heteroduplex Analysis................................................................44
5.1 Introduction to Heteroduplex Analysis ......................................................44
5.2 Reagent Preparation................................................................................45
5.3 Gel Volumes ............................................................................................47
5.4 Sample Preparation .................................................................................48
5.5 Temperature Controller............................................................................48
5.6 Adding the Running Buffer .......................................................................49
5.7 Assembling the Heteroduplex Analysis Gel Sandwich .............................50