Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 68)

Coomassie Blue Destain
Reagent
Amount Final Concentration
Methanol 400 ml 40%
Acetic acid, glacial 100 ml 10%
dH
2
O 500 ml
Total volume 1,000 ml
Mix and store at room temperature.
7.3 Gel Volumes
The table below provides the required volume for the gel size and spacer thickness.
Spacer Thickness 20 x 20 cm Gel
0.75 mm 30 ml
1.00 mm 40 ml
7.4 Sample Preparation
1. Dilute samples, controls, and protein marker (such as Bio-Rad pre-stained markers) 1:2
with Laemmli sample buffer.
Note: Add 10 µl
b-mercaptoethanol to 590 µl Laemmli buffer just before use. This solution
is good for 1 day only.
2. Heat the samples at 95–100°C for 5 minutes.
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