Bio-Rad PowerPac™ HV High-Voltage Power Supply - Manual (Page 26)

4.3 IEF Methods Mode
The IEF Method mode is used to create, run and store Isoelectric focusing (IEF) methods.
The method list stores up to nine methods, with up to nine steps each. Special programming
options such as voltage ramping and run delay in the first step, allow better control during
IEF separations. These methods may also be transferred to or from a PDA, through the
PowerPac IR port as described in Section 5.
Fig. 9. Menu Overview for IEF Methods
IEF METHOD LIST
* pH 3-10
IEF
***
EMPTY…
_________
___________________________
OPEN DELETE NEW
METHOD: PH 3-10
*S1 100V
Ê 00:30 HRS
S2 800V Ê 05:30 HRS
S3 …EMPTY….________
METHOD DELETE (SAVE)
S1/1 VOLT PROFILE
2000 V
V-SLOPE
LINEAR
TIME: 0:00 HRS
V-MAX V-SLOPE (DELAY)
METHOD:UNTITLED
* S1 ****EMPTY****
____________________
METHOD
S1/1 EDIT TIME
2000 V
0.10 mA
0.1 W
TIME: 00:30 HRS___________
HRS VHRS
NAME: xxxxx
__________________
OK CANCEL
EDIT
EDIT
S1/1 EDIT LIMITS
2000 V
0.00 mA
0.1 W
TIME: 0:00 HRS
V-MAX LIMITA LIMITW
EDIT EDIT
EDIT
SELECT MODE
METHODS
MANUAL IEF BASIC
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PowerPac HV – Instruction Manual