Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 52)

"Changing Cutting Tips".
7.3 Changing Cutting Tips
Turn off the spot cutter and move the cutting head to the empty microtiter plate area. Use the two
cutting tip wrenches (spanners) supplied with the system. One wrench is used to hold the cutting
tip holder nut located on the cutting shaft. The other wrench holds the cutting tip. Turn the tip
wrench clockwise to remove the tip. A new tip can then be inserted and turned counter-clockwise
to install. Use the same wrenches to tighten the new cutting tip.
7.4 Cutting Tip Height
• Place the cutting mat and protective gel sheet on the cutting platform.
• Click the "Set Cutting Tip Height" icon in the Set up area of the "Basic Excision Tool"
dialog box to move the cutting head to the middle of the cutting platform and the "set
cutting height" dialog box appears on the screen.
• From the "Set Cutting Tip Height" dialog box, use the "lower head" command to assess
how far down the cutting tip goes. The cutting tip should lightly push down onto the
protective gel sheet. If the cutting tip is too high off the cutting mat or too far down into
the cutting mat adjust the cutting tip height using the following steps:
1. Loosen the set screw in the gold Spindle Hexagonal Screw at the top of the cutting
shaft. This set screw is accessed through the hole in the head cover, as indicted in
the photo, Figure 45a. If you are having difficulty in finding the set screw inside, take
the black cover off by removing the two 2.5 mm screws holding the cover on. You
will then see the cutting head as Figure 45b.
Fig. 45a. Set cutting tip height with cover Fig. 45b. Cutting head.
on.
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