Bio-Rad PDQuest 2-D Analysis Software User Manual
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5.2
Plastic and Glass Backed Gel Preparation ..........................................34
5.3
Using PDQuest Basic Excision Tool Software ....................................34
5.3.1
Step 1: Acquire Image ..........................................................35
5.3.2
Step 2: Specify Cuts ..............................................................38
5.3.3
Step 3: Set Cut Options ........................................................40
5.3.4
Step 4: Set Wash Options......................................................41
5.4
Start Spot Excision ............................................................................42
5.4.1
Begin/Resume and Pause......................................................42
5.4.2
Re-cuts in Basic Excision Tool ..............................................42
5.5
Confirm Cuts ......................................................................................46
Section 6
Accessories ....................................................................................47
6.1
Use of Gel Sheets and Gel Positioning ..............................................47
6.2
Common Re-order Parts ....................................................................48
Section 7
Maintenance....................................................................................48
7.1
General Maintenance and Fuses ........................................................48
7.2
Cutting Tip Cleaning ..........................................................................48
7.3
Changing Cutting Tips ........................................................................49
7.4
Set Cutting Tip Height ........................................................................49
7.5
Adjusting the Cutting Carriage ............................................................50
7.6
Ejection Pin ........................................................................................51
Section 8
Troubleshooting ..............................................................................52
8.1
Communication Error ..........................................................................52
8.2
Image Quality......................................................................................53
8.3
Lights
..............................................................................................53
8.4
Spot Cutting and Pickup ....................................................................54
8.5
Technical Service ................................................................................54
Section 9
Warranty ..........................................................................................55
Appendix A Spot Cutter – Front ........................................................................56
Appendix B Working Surface ............................................................................57
Appendix C Spot Cutter – Back ........................................................................58
Appendix D PDQuest Version 7.0 and 7.1 ......................................................59
Glossary ..................................................................................................................61
System Records ....................................................................................................62