Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 35)

Set Cutting Tip Height
Fig. 30. Set cutting tip height button.
• Set Cutting Tip Height button to adjust the distance the cutting tip drops down to cut a
spot. The adjustment dialog box will open, and the cutting tip will move to the center of
the gel.
• Lower Tip to drop the cutting tip to its lowest position. In this position, manually adjust the
cutting tip height as described in Section 2.12 until it presses lightly against the surface of
the cutting mat.
• Raise Tip raises the cutting tip
• Test Cut performs a test cut in the center of the gel.
• Click on Done to accept the settings and return the cutting tip to home position.
Spot Cutter Calibration
Fig. 31. Calibrate cutter button.
Before you can begin excising spots, you must calibrate the spot cutter. This button starts the
calibration procedure.
Move Cutter Head
Fig. 32. Move cutter head buttons.
The two boxes with diagonal arrows inside them move the cutting head to the lower left corner
position, or the upper right corner position.
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