Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 2)

Table of Contents
Section 1 Introduction ......................................................................................1
1.1 General Description ..............................................................................1
1.2 Manual Guidelines ................................................................................1
1.3 System Components ............................................................................1
1.4 Safety Information ................................................................................2
1.5 Caution ................................................................................................3
1.6 Moving the Spot Cutter ........................................................................3
1.7 Computer Recommendations ..............................................................3
1.8 Cutting Excision Sizes ..........................................................................3
1.9 Voltage Specifications ..........................................................................3
1.10 System Description ..............................................................................4
Section 2 Installation ........................................................................................8
2.1 Overview ..............................................................................................8
2.2 Installing PDQuest Software..................................................................8
2.3 Install PCI Card ....................................................................................9
2.4 Spot Cutter Setup ................................................................................9
2.5 Camera Installation ............................................................................10
2.6 Camera Lens Aperture Settings ..........................................................12
2.7 Cable Setup........................................................................................12
2.8 Power ON Startup Sequence ............................................................13
2.9 Spot Cutter Model Number Selection ................................................14
2.10 Camera Focus ....................................................................................15
2.11 Camera Alignment ..............................................................................17
2.11.1 Camera Rotation Alignment ..................................................17
2.11.2 Front/Back Centering of the Camera ....................................18
2.11.3 Lateral Centering of the Camera ............................................18
2.12 Cutting Tip Height ..............................................................................20
2.13 Microtiter Plate (MTP) Calibration ........................................................21
2.14 Wash Well Position Calibration............................................................23
2.15 Fluorescent Enclosure Setup ..............................................................23
Section 3 System Calibration ........................................................................27
3.1 Switch to White Light Imaging ............................................................27
3.2 Prepare Coomassie Stained PVDF Membrane....................................27
3.3 Calibration of X, Y Coordinates ..........................................................28
Section 4 Software Features Description....................................................30
4.1 Spot Cutter Settings ..........................................................................30
Section 5 Spot Excision ..................................................................................33
5.1 Free Gel and Blot Preparation ............................................................33