Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 20)

16
Fig. 4. Linearity of Oriole stain. A dilution series of carbonic
anhydrase was run on a Criterion gel, stained with Oriole stain,
and imaged on the Molecular Imager VersaDoc MP 4000 imaging
system. Fluorescence associated with the carbonic anhydrase
band was plotted following background subtraction.
Lo
g (
flu
or
es
ce
nc
e)
,
ar
b
itr
ar
y u
ni
ts
3.4. Dynamic Range
Oriole fluorescent gel stain has a dynamic range of up to
three orders of magnitude. This allows protein quantitation
in samples of varying concentration over a wide range of
relative abundance.
4.5
4
3.5
3
2.5
2
0 0.5 1 1.5 2 2.5 3
Log carbonic anhydrase, ng