Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 14)

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2. Stain for 90 min for maximum sensitivity.
3. Cover the tray to exclude light if the gel is stained
longer than 90 min.
NOTE: Best results are obtained if the gels are left in
staining solution no longer than 2 hr.
4. Transfer gel to water prior to imaging. This step
prevents exposure of the imaging equipment
to moderately corrosive staining solution.
NOTE: Destaining is not necessary. Staining intensity
persists when the gel is stored in water.
Stained gels can be stored in water for up to 6 months
and imaged without significant loss of sensitivity if
protected from light and stored at 2–8°C.