Bio-Rad 2-D Electrophoresis Workflow User Manual
Page 19
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15
3.2. Compatibility With Mass Spectrometry
Oriole fluorescent protein gel stain is fully compatible with
downstream proteolysis and mass spectrometric analysis.
3.3. Protein-to-Protein Variability
Oriole fluorescent gel stain will stain most proteins
and exhibits little protein-to-protein variability in
staining intensity.
Fig. 3. Image of the gel
stained with Oriole stain,
adjusted to show the limit
of sensitivity. The image
from the previous figure
was inverted, cropped to
show protein loads ≤ 4 ng,
and adjusted to show the
limit of sensitivity.
4
2
1 0.5 0.25 0.125 ng/band
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