Bio-Rad 2-D Electrophoresis Workflow User Manual
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13
Manufacturer Imaging system
Recommended settings
UVP
BioDoc-It,
Midrange (302 nm)
VisiDoc-It,
UV excitation with ethidium
DigiDoc-It,
bromide red emission filter
MultiDoc-It,
Photo Doc-It,
BioSpectrum,
EC3
Carestream
Gel Logic
UV trans excitation
with 590 nm bandpass
(ethidium bromide)
emission filter
Kodak
2200, 4000MM,
306 nm UV excitation
4000MM Pro,
with ethidium bromide
4000R, 4000R Pro
standard orange (600 nm)
emission filter
Syngene
G:Box, InGenius,
Midrange UV excitation
U:Genius
with ethidium bromide
emission filter
Biometra
BioDocAnalyze
Midrange (312 nm) UV
excitation with ethidium
bromide emission filter
Imaging systems using laser light excitation or other visible light
source excitation are not recommended for imaging gels stained
with Oriole fluorescent gel stain. These include PharosFX
™
system
(Bio-Rad), Typhoon, Storm, and Ettan DIGE Imager (GE Healthcare),
Odyssey (Li-Cor), FLA (Fuji), and FM Bio (Hitachi) systems.