Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 12)

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Molecular weight standards that have been prestained
with a visible dye such as Precision Plus Protein
™ All Blue,
Dual Color or Kaleidoscope ™ prestained standards do
not stain with Oriole fluorescent gel stain and cannot be
imaged by fluorescence in gels stained with Oriole stain.
We recommend the use of unstained protein standards on
gels to be stained with Oriole stain.
2.2. Stain Solution Preparation
The 200 ml and 1 L configurations are provided ready
to use.
The 5 L kit comprises 5 individual 1 L bottles, each
containing 590 ml of Oriole fluorescent gel stain diluent,
and a single bottle containing 50 ml of Oriole gel stain
concentrate. Staining solution (1x) is prepared as follows.
n
To a 1 L bottle holding 590 ml of diluent,
add (in sequence):
– 400 ml methanol (reagent grade)
– 10 ml of Oriole fluorescent gel stain concentrate
n
Mix well by shaking
n
Stain is now ready to be used