Bio-Rad Silver Stains - Manual (Page 8)

2) Dodeca stainer with less than 12 trays. Although the provided volumes of the concentrates are
optimized to stain 12 PROTEAN gels (or 24 Criterion gels) in a Dodeca stainer, fewer gels also may be
stained. However, because of inherent dead volumes in the Dodeca stainer (as with most instruments),
a slightly higher solution volume per tray ratio is required for optimal results. Using Table 3, the required
working solution volumes are calculated by taking the number of staining trays used (not including the
cover tray) multiplied by the volume listed in the appropriate “Per Tray” column, and then adding the
corresponding volume listed under the adjacent “Plus” column. For example, six PROTEAN Plus gels
stained using six trays in the large Dodeca stainer require (6 trays x 750 ml) + 1.5 L, for a total volume of
6.0 L for each working solution. Silver staining with fewer than four trays is not recommended.
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