Bio-Rad Silver Stains User Manual

Page 7

Working Solution Volumes

For best results, prepare each working solution no more than 24 hr before use. Add the image developer
concentrate to the developing working solution within 15 minutes of use. Note: The volume corresponding
to water (Table 2, 3 and 4) indicates the final volume of the working solution.

Please refer to the appropriate section below to determine the proper working solution volumes for staining
1) 12 PROTEAN gels in a Dodeca stainer, 2) fewer than 12 gels in a Dodeca stainer, or 3) individual gels in a
user-provided staining container.

Dodeca stainer with 12 trays. When using the full capacity of the Dodeca stainer, prepare each
working solution as directed by Table 2. Please note that the cover tray is not counted.

Table 2. Dodeca stainer solution volumes.

Working Solutions

Reagents

Small Dodeca Stainer

Large Dodeca Stainer

12 Trays

Fixing

Ethanol

2.8 L

4.0 L

Glacial acetic acid

700 ml

1.0 L

Water

To 7 L

To 10 L

Sensitizing

SC (10×)

700 ml

1.0 L

BRC (100×)

70 ml

100 ml

Ethanol

2.1 L

3.0 L

Water

To 7 L

To 10 L

Staining

SRC (50×)

140 ml

200 ml

Water

To 7 L

To 10 L

Developing

DBC (10×)

700 ml

1.0 L

IDC (5,000×)

1.4 ml

2.0 ml

BRC (20,000×)

350 µl

500 µl

Water

To 7 L

To 10 L

Stopping

Glacial acetic acid

350 ml

500 ml

Water

To 7 L

To 10 L

4110150B.qxd 7/31/2003 1:39 PM Page 5