Bio-Rad Silver Stains User Manual
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Section 1
Introduction
The Dodeca
™
silver stain kit is an easy-to-use kit for the detection of nanogram levels of proteins in
polyacrylamide gels. The kit is formulated with concentrated aqueous solutions that enable the user to
quickly prepare volumes of working solutions that may be easily tailored to the number of gels to be stained.
Furthermore, the kit is designed to be compatible with the high-throughput Dodeca stainer by facilitating
reagent preparation and instrument cleaning.
The Dodeca silver stain kit is based on the method described by Sinha et al. (2001) in which protein-bound
silver ions are chemically reduced to form visible metallic silver. Additionally, no protein-modifying
glutaraldehyde is used, and only minimal concentrations of the reducing agent, formaldehyde, which is
required for silver stain development. Consequently, this procedure yields silver-stained proteins that are
compatible with mass spectrometry for protein identification analysis.
Reference
Sinha P et al., A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins
after two dimensional electrophoresis, Proteomics 1, 835–840 (2001)
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