Bio-Rad Silver Stains User Manual

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Section 5
Troubleshooting Guide

Problem

Cause

Solution

Background dark or uneven

Agitation too gentle.

Increase agitation speed during
gel incubation and ensure gel is
completely submerged.

Dodeca stainer or staining

Thoroughly clean Dodeca stainer

containers contain residue from

or containers with soap and

prior cleaning or staining runs.

water. Rinse only glass
containers with 50% nitric
acid, followed by thorough
water rinse to remove the acid.

Fingerprints or powder from

Use powder-free laboratory

gloves cause staining artifacts.

gloves.

Interfering components (glycine,

Increase fixing time or use

SDS, etc.) not completely

multiple fixing cycles to

removed from gel.

completely wash interfering
compounds from gel.

Sensitizing working solution

Prepare new sensitizing

improperly prepared.

solution with BRC.

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