Bio-Rad Silver Stains - Manual (Page 14)

Problem Cause Solution
Low protein staining sensitivity or Development terminated Increase the development
no staining (cont.) prematurely. time. Protein stain may be
developed up to 30 min
with a minimal increase
in background.
Negative protein staining (light bands Protein sample overloaded. Reduce the amount of
or spots on a darker background) protein loaded.
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