Bio-Rad Gel Doc™ EZ System User Manual
Page 7
Image Lab Software for Obtaining Stain-Free Gel and Blot Images 3
6. In the Image Exposure pane, select from one of the following options:
a. Auto Exposure — this setting estimates an optimal exposure time and ensures the best use of the
dynamic range.
i. Intense Bands — optimizes exposure for all bands.
ii. Faint Bands — a longer exposure time is used to make faint bands more visible, but more
prominent bands might be overexposed.
b. Manual Exposure — use this setting to manually override automated imaging. This setting is often
used to duplicate an exposure time in order to compare band intensities from different gels.
7. In the Display Options pane, keep the default settings. If using the Gel Doc EZ, proceed to step 10.
8. Select Position Gel. The acquisition pane will now display a live view of the gel.
9. Make sure the gel is correctly positioned in the viewing area. Adjust the zoom if necessary and/or
manually align the gel.