Bio-Rad Gel Doc™ EZ System - Manual (Page 6)

2 Image Lab Software for Obtaining Stain-Free Gel and Blot Images
4. In the Application pane, click Select and then Protein Gels > Stain Free Gel. Now select the gel
activation, depending on the application.
a. 1 min activation provides sufficient UV activation for gels used in western blots.
b. 2.5 min activation provides a good balance between time required for UV activation and signal
intensity.
c. 5 min activation provides maximum signal intensity.
5. In the Imaging Area pane, select from the list of Bio-Rad gels or enter the image area dimensions
manually. This option is not necessary when using the Gel Doc EZ, as the entire area is imaged
automatically.