beautypg.com

Bio-Rad Gel Doc™ EZ System User Manual

Page 14

background image

10 Image Lab Software for Obtaining Stain-Free Gel and Blot Images

Notes and Tips

If desired, stain the gel with any TGX-compatible stains after stain-free imaging. Certain stains eliminate
detection capability if used prior to imaging.

Wet the UV transilluminator with 2–3 ml of water prior to gel placement in order to avoid tearing.

If uneven stain-free development is observed, briefly rinse the gel with water after electrophoresis. However,
prolonged rinsing may affect the intensity of the stain-free signal.

Since gels are transparent, what shows in the Live View window of ImageLab for ChemiDoc MP, XR+, and
XRS+ instruments are white dots that define the edges of the gel.

For more detailed instructions, refer to the Gel Doc EZ Stain-Free Sample Tray Instruction Manual
(#10019634), the ChemiDoc MP System with Image Lab Software Instruction Manual (#10022469) and the
Mini-PROTEAN

®

Precast Gels Instruction Manual and Application Guide (#1658100). These bulletins, as well

as a library of other manuals, guides, and technical information are available at www.bio-rad.com.