Bio-Rad S3™ Cell Sorter - Manual (Page 25)

Certification Procedure
3. Inject PAO in such a manner to allow for uniform particle distribution across the entire face
of the HEPA filter.
4. Set the 100% concentration on the photometer by sampling in the work area or by
calculating from the aerosol generator output rating using the calculated or measured
exhaust CFM volume of the enclosure (section 4.1.4).
5. Acceptance: Minimum upstream concentration should be >10 µg/L.
6. Insert photometer probe in the discharge of exhaust fan or duct section. Sample and
record the gross leakage value.
7. Acceptance: Sustained aerosol penetration must not exceed 0.01% of the upstream
concentration, unless otherwise specified.
Airflow Smoke Patterns, Visual Containment
1. Pass the smoke along the exterior perimeter of the 11 x 15 inch sample access opening,
approximately 2 inches from the opening. Inspect for any reflux of smoke into the
laboratory work environment.
2. Pass smoke along the entire length on the exterior side the of the 7 mm slot located at the
bottom of the enclosure.
3. Acceptance: Smoke shall be drawn into the enclosure, and once drawn into the enclosure,
no smoke shall be seen escaping from the enclosure.
Certification
Passing results: Complete form below and attach certification sticker as shown (Figure 13).
Corrective action: If any of the above tests do not meet the requirements, refer to the S3
biosafety system Class I service manual.
References
SEFA 1 Recommended Practice, latest revision, section: Robotic Enclosure.
ASME N510 latest revision
IEST-RP-CC034 latest revision
Instruction Manual | 15
Fig. 13. Apply the onsite certification certificate onto the air generator.