Bio-Rad SsoAdvanced™ Universal Probes Supermix - Manual (Page 3)

SsoAdvanced ™ Universal Probes Supermix Instruction Manual | i
Table of Contents
Sso7d Fusion Enzyme Technology iii
Educational Resources iv
Reagent Evaluation and Comparison Tutorials iv
Protocol
1
Sample Preparation Considerations 1
RNA Samples 1
RNA Integrity and Purity 1
DNA Samples 2
Plasmid Samples 2
Assay Design Considerations 3
Some Key Design Considerations 3
Multiplex Assays Design Considerations 4
Procedure 6
Reaction Mix Preparation and Thermal Cycling Protocol 6
Real-Time PCR Validation for Gene Expression Experiments 7
Determining the Optimal Reference Gene 7
Determining the Dynamic Range of the Reverse Transcription Reaction
9
Determining the PCR Efficiency 11
Troubleshooting Guide
14
Ordering Information
22