Bio-Rad SsoAdvanced™ Universal Probes Supermix - Manual (Page 16)

10 | SsoAdvanced ™ Universal Probes Supermix Instruction Manual 10 |
6. Evaluate the data. Follow the guidelines in this manual (page 14–15) for setting the baseline
and threshold prior to analyzing the data. Figure 6 illustrates the most common results from
the experiment and how to interpret the data.
Fig. 5. A recommended plate layout.
Reference
gene
Medium
expressor
Low
expressor
High
expressor
4. Dilute the cDNA as needed to perform real-time PCR reactions using a minimum of two
genes — reference and low expressing. However, it is recommended to evaluate four genes
— reference, low, medium, and high expressing.
5. Prepare the real-time PCR plate (Figure 5) and cycle according to the recommended protocol.