Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 9)

QX100 Droplet Generator Instruction Manual QX100 Droplet Generator Instruction Manual | 3
Chapter 1 QX100 Droplet Generator Chapter 1 QX100 Droplet Generator
Table 1.2. Additional materials required for droplet generation.
Component Recommended Catalog #
PCR supermix (required) ddPCR supermix for probes 186-3010, 186-3026,
186-3027, 186-3028
One-Step RT-ddPCR kit for probes 186-3021, 186-3022
Control Buffer control kit 186-3052
Droplet generator oil Droplet generator oil (10 x 7 ml) 186-3005
Pipets 20 μl pipet for sample loading Rainin L-20
50 μl pipet for droplet transfer Rainin L-50, L8-50
8-channel, 200 μl pipet for oil Rainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough For droplet generator oil Thermo 95128095
Foil plate seals Easy Pierce foil plate seals Thermo AB-0757
Plate sealer Eppendorf 951023078
8-cap strips Any
1.3 Installation and General Operation
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Connect the QX100 droplet generator to a power source using only the power cord provided. Ensure the
ground is reliably connected before plugging in the instrument
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Leave 10" (5 cm) space clear behind and 5" (2.5 cm) clear to the right and left for proper ventilation
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Power on the droplet generator by plugging it in. The status indicator turns solid green to indicate power is on
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Open and close the instrument by pressing the button on top of the green lid
U.S. Standard power cord set with grounded plug
(Type 5-15P) and C5 connector (10 A/125 V) Power supply to 5 mm DC power jack inlet