Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 14)

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Chapter 2 Droplet Generation
QX100 Droplet Generator Instruction Manual
5. Using a multichannel pipet, fill each oil well (bottom row) with 70 μl DG oil from the reagent trough.
Filling the oil wells with droplet generator oil.
6. Hook the gasket over the cartridge holder using the holes on both sides. The gasket must be securely
hooked on both ends of the holder; otherwise, sufficient pressure will not build up for droplet generation.
Correct placement of the gasket over the cartridge holder.
7. Place the cartridge holder into the QX100 droplet generator and initiate droplet generation:
a. Open the instrument by pressing the button on the green top
b. Place the cartridge holder into the instrument. When the holder is in the correct position, both the
power (left light) and holder (middle light) indicator lights are green (Table 2.1)
8. Press the button on the top again to close door. This initiates droplet generation: a manifold positions itself
over the outlet wells, drawing oil and sample through microfluidic channels where droplets are created.
Droplets flow to the droplet well, where they accumulate. The droplet indicator light (at right) flashes green
after 10 sec to indicate droplet generation is in progress.