Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 17)

QX100 Droplet Generator Instruction Manual | 11
Chapter 2 Droplet Generation
QX100 Droplet Generator Instruction Manual
Chapter 2 Droplet Generation
2. Seal the PCR plate wells with foil immediately after transferring droplets to avoid evaporation. Use
Easy Pierce foil plate seals (compatible with the needles in the QX100 droplet reader) and a compatible
thermal plate sealer.
a. Allow the plate sealer to warm up for at least 10 min before use.
b. Place the foil seal over the plate and place the plate in the plate sealer.
c. Press the sealer firmly against plate for 3 sec, then rotate the plate 180° and seal for another 3 sec.
d. Check that all the wells in the plate are sealed. The depressions of the wells should be visible on the
foil. Once sealed, the plate is ready for thermal cycling.
3. Once droplets are removed, press the latches on the DG8 cartridge holder to open it. Remove the empty
DG8 cartridge and discard it.
Eppendorf plate sealer (left) and a sealed 96-well plate (right).
Begin thermal cycling (PCR) within 30 min of sealing the plate, or store the plate at 4°C for up to 4 hr prior
to thermal cycling. Refer to the supermix product inserts for cycling conditions.