Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 12)

QX100 Droplet Generator Instruction Manual 6 |
Chapter 2 Droplet Generation
2.2 Operation of the QX100
™
Droplet Generator
1. Insert the DG8 cartridge into the holder with the
notch in the cartridge at the upper left of the
holder:
a. Open the cartridge holder by pressing the
latches in the middle.
b. Slide the DG8 cartridge into the right half of
the holder, then drop it down.
c. Press the halves of the holder together to
snap it closed.
Inserting the DG8 cartridge into the cartridge holder.
The QX100 droplet generator prepares droplets for eight samples at a time. Droplet generation takes
2 minutes for each set of eight samples (20–30 minutes for a 96-well plate).
■ ■
All 8 sample wells in the DG8 droplet generator cartridge must contain sample (or 1x buffer
control), and all 8 oil wells must contain droplet generator oil
■ ■
Do not load the DG8 cartridge with sample or oil unless it is already inserted in the holder
Press
Press to open Slide to close
Notch
Top wells (droplets)
Middle wells
(20 μ l sample)
Bottom wells (70 μ l oil)
DG8 cartridge