Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 7)

QX100 Droplet Generator Instruction Manual | 1
QX100
™
Droplet
Generator
1
1.1 Introduction
The QX100 ™ Droplet Digital ™ PCR (ddPCR ™ ) system performs digital PCR with
unrivaled accuracy and precision. The system consists of two instruments, the
QX100 droplet generator and the QX100 droplet reader, and their associated
consumables. The QX100 droplet generator partitions samples into 20,000
nanoliter-sized droplets, and after PCR on a thermal cycler, droplets from each
sample are analyzed individually on the QX100 droplet reader. PCR-positive and
PCR-negative droplets are counted to provide absolute quantification of target
DNA in digital form.
The ddPCR system lets you:
■ ■
Detect rare DNA target copies with unmatched sensitivity
■ ■
Determine copy number variation with unrivaled accuracy
■ ■
Measure gene expression levels with precision
Applications and uses include:
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Copy number variation
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Rare sequence detection
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Mutation detection
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Gene expression analysis
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miRNA analysis
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Next-generation sequencing sample quantification
This manual covers use of the QX100 droplet generator and preparation
for PCR. For information on the QX100 droplet reader, please refer to
bulletin 10026321.