Bio-Rad Peelable Foil Heat Seal - Manual (Page 19)

Compatible PCR Plastics
and Heat Seals
5
13
Compatible PCR Plastics and Heat Sealing Films
Product Catalog Number Key Features Compatibility
Optically clear
heat seal 181-4030
n
Validated for real-time PCR
n
Peelable
PP, COC, PE*, PS
Permanent clear
heat seal 181-4035
n
Strongest heat sealing option
n
Ideal for water bath cycling
n
Validated for PCR**
PP
Pierceable foil
heat seal 181-4040
n
Validated for use with the
QX100
™ Droplet Digital ™ PCR
system
n
Validated for PCR
PP, PS*
Peelable foil
heat seal 181-4045
n
Ideal for sample storage
n
Validated for PCR
PP, COC*
* Limited and variable compatibility based on plastic composition. ** Not recommended for real-time
PCR. PP, polypropylene; COC, cyclic olefin copolymer; PE, polyethylene; PS, polystyrene.
Table 4. Heat sealing film comparison chart.
PCR Plate Catalog
Number
Number
of Wells
Well
Color
Optically
Clear Heat
Seal
Permanent
Clear Heat
Seal
Pierceable
Foil Heat
Seal
Peelable
Foil Heat
Seal
Hard-Shell ®
384-well
standard
HSP-3xxx 384 Clear
White 175, 3
167, 3 175, 3
167, 3 170, 3
170, 3 185, 4
185, 3
Hard-Shell
384-well 480 HSR-4xxx 384 Clear
White 175, 3
167, 3 175, 3
167, 3 170, 3
170, 3 185, 4
185, 3
Hard-Shell low-
profile 96-well
skirted HSP-9xxx 96 Clear
White 185, 3
180, 3 180, 3
180, 3 175, 3
175, 3 180, 3
185, 5
Hard-Shell high-
profile 96-well
semi-skirted HSS-9xxx 96 Clear
White 170, 3
185, 3 165, 3
180, 3 170, 3
175, 3 185, 3
185, 3
Multiplate ™
low-profile 96-
well unskirted
MLL-9xxx 96 Clear
White 180, 3
180, 3 180, 3
160, 3 175, 3
175, 3 185, 5
185, 3
Multiplate high-
profile 96-well
unskirted MLP-9xxx 96 Clear
White 175, 3
170, 3 150, 3
165, 3 175, 3
175, 3 185, 3
185, 3
iQ ™ 96-well 223-9441 96 Clear 180, 3 180, 3 185, 3 185, 5
Table 5. Suggested sealing guidelines for heat seals. Use these sealing times and temperatures for
initial plate sealing. Optimize sealing parameters if necessary.
Temp°C, sec