LaMotte AM-21 Water Pollution Detection Outfit - Manual (Page 40)

Overhead Projection Demonstration
Quantity Contents Code
60 mL *VM Phosphate Reagent *4410-H
5 mL Reducing Reagent 6405-C
1 Test Tube, 5.0 mL 0230
1 Pipet, 1.0 mL 0354
1 Pipet, unmarked 0352
1 Demonstration Stage, six cell 1038
1 Spatula 0691
WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD
or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.
Procedure
1. Place the demonstration stage (1038) on the overhead projector and
turn on the projector light.
2. Fill test tube (0230) to the 5 mL line with sample water and transfer to a
cell on the demonstration stage (1038).
3. Use the 1.0 mL pipet (0354) to add 1.0 mL of the *VM Phosphate
Reagent (4410). Stir the contents of the cell with the spatula (0691).
Wait five minutes. A light yellow color may appear.
4. Use the pipet (0352) to add 3 drops of the Reducing Reagent (6405).
Stir the contents of the cell.
5. If Phosphate is present, a blue color will form. High concentrations of
Phosphates will produce a deep blue color. Lower concentration will
produce varying shades of light blue and a faint blue tint will indicate the
presence of trace quantities.
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