LaMotte AM-21 Water Pollution Detection Outfit - Manual (Page 15)

Overhead Projection Demonstration
Quantity Contents Code
100 Hardness Reagent #6 Tablets 4484-J
15 mL *Hardness Reagent #5 *4483-E
15 mL *Sodium Hydroxide Reagent
w/Metal Inhibitors
*4259-E
50 Calcium Indicator Tablets T-5250-H
30 mL Hardness Reagent #7 4487DR-G
1 Test Tube, 12.9 mL, w/cap 0608
1 Direct Reading Titrator, 0-200 Range 0382
1 Demonstration Stage, six cell 1038-P
1 Spatula 0691
WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD
or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.
Read the LaMotte Direct Reading Titrator Manual before proceeding.
Procedure
1. Place the demonstration stage (1038) on the overhead projector and
turn on the projector light.
2. Fill the test tube (0608) to the 12.9 mL line with sample water and
transfer to a cell on the demonstration stage (1038). Repeat this
operation so that four cells of the demonstration stage are filled. One cell
will be used for the Total Hardness test, one cell will be used for the
Calcium Hardness test and the other two cells will be used as controls.
Number the cells 1, 2, 3 and 4.
3. To cells 1 and 2, add 5 drops of *Hardness Reagent #5 (4483) and mix.
Add 1 Hardness Reagent #6 Tablet (4484) and swirl until tablet is
disintegrated. A red color will develop.
4. Fill the Direct Reading Titrator (0382) with the Hardness Reagent #7
(4487). Add one drop at a time to cell 2 until the sample color changes
from red to clear blue. Stir the sample after the addition of each drop.
Read the test result directly from the scale where the large ring on the
Titrator meets the Titrator barrel. Record the result as ppm Total
Hardness.
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