Metrohm 930 Compact IC Flex Oven/ChS/PP/Deg - Manual (Page 62)

3.20 Connecting the separation column
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930 Compact IC Flex Oven/ChS/PP/Deg (2.930.2360)
2 Installing the input of the separation column
There are 3 possibilities:
￭ Attaching the bottom end of the separation column directly to
the guard column.
or
￭ If the guard column is connected to the separation column using
a provided connection capillary, connect the bottom end of the
separation column to the guard column's output capillary using
the provided PEEK pressure screw (6.2744.070).
or
￭ If no guard column is used (not recommended), connect the col-
umn input capillary to the input of the separation column using a
short pressure screw (6.2744.070).
3 Rinsing the separation column
￭ Place a beaker under the separation column's outlet.
￭ Start manual control in MagIC Net and select the high-pressure
pump: Manual
▶ Manual control ▶ Pump
– Flow: in accordance with column leaflet
– On
￭ Rinse the separation column with eluent for approximately 10
minutes.
￭ Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
4 Removing the coupling
￭ Remove the coupling (6.2744.040) from the column output capil-
lary.
5 Installing the output of the separation column
￭ Fasten the column output capillary to the upper end of the sepa-
ration column using a PEEK pressure screw (6.2744.070).
6 Inserting the separation column
￭ Insert the separation column with chip into the column holder
until you hear it snap in place.
The separation column is now detected by MagIC Net.