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Tl rci™: the deflection mirror – Leica Biosystems M205A User Manual

Page 81

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Leica M series

Manual

81

TL RCI™: The Deflection Mirror

Inverted world?

Depending on the properties of the specimen
(refractive index of the environment) and the
perception of the viewer, it is possible that
the switches described below for positive and
inverted relief contrast are to be operated in
reverse. This means that the lower switch, rather
than of the upper switch, controls inverted relief
contrast and vice-versa.

Tilted mirror

The built-in mirror features one flat and one
concave side and can be rotated and moved.
The concave side has been specially designed
for the optical requirements of objectives with a
high numerical aperture. The black rotary knob
on the left side of the transmitted-light base
can be used to rotate the built-in path-folding
mirror and move it forwards/back.

The concave cutout on the handle indicates the
concave side of the mirror, allowing intuitive
operation at any time without eye contact.

The angle of light incidence in the specimen
plane changes depending on the tilt and posi-
tion of the mirror. As a result, switching between
transmitted light bright field, oblique illumina-
tion and dark field-like illumination is possible.

Functions of the rotary knob

The rotary knob (1) fulfills the following tasks:

Turning the deflection mirror from the flat

to the concave side.

Slight tilting to guide the light beam

through the specimen plane at a steeper or
flatter angle

Moving the deflection mirror (forwards/

back)

1

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