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Instruction manual V 1.4 – 04/2010
Table of contents
1.
Important information ................................................................................................................ 6
1.1
Symbols used in the text and their meaning .......................................................................................... 6
1.2
Instrument type ............................................................................................................................................. 6
1.3
Designated use ............................................................................................................................................. 6
1.4
Qualification of personnel .......................................................................................................................... 6
2.
Safety ............................................................................................................................................ 7
2.1
Safety instructions ....................................................................................................................................... 7
2.2
Dangers, warnings and cautions .............................................................................................................. 7
2.3
Safety features ............................................................................................................................................ 11
2.3.1
Locking the handwheel ............................................................................................................................. 11
2.3.2
Knife guard ................................................................................................................................................... 11
3.
Instrument components and specifications ........................................................................ 12
3.1
Overall view - instrument parts ................................................................................................................ 12
3.2
Technical data ............................................................................................................................................ 14
4.
Setup ........................................................................................................................................... 16
4.1
Installation site requirements .................................................................................................................. 16
4.2
Standard delivery - packing list ............................................................................................................... 17
4.3
Unpacking and installation ...................................................................................................................... 18
4.4
Fastening the handwheel ......................................................................................................................... 19
4.5
Electrical connection ................................................................................................................................ 20
4.6
Prior to operation ........................................................................................................................................ 20
5.
Operation .................................................................................................................................... 22
5.1
Control panel ............................................................................................................................................... 22
5.2
Controller ...................................................................................................................................................... 23
5.3
Setting user-definable parameters ......................................................................................................... 24
6.
Daily work with the instrument ............................................................................................. 26
6.1
Specimen freezing ...................................................................................................................................... 26
6.1.1
Quick-freeze shelf ....................................................................................................................................... 26
6.2
Inserting the specimen discs .................................................................................................................. 26
6.2.1
Fixing the specimen discs in the specimen head ............................................................................... 27
6.2.2
Specimen orientation ................................................................................................................................ 27
6.3
Inserting the knife or blade into the knife holder ............................................................................... 27
6.4
Sectioning / trimming ................................................................................................................................. 28
6.4.1
Moving the specimen with the coarse / precision feed .................................................................... 29
6.4.2
Section thickness setting ......................................................................................................................... 29
6.5
Defrosting ..................................................................................................................................................... 30
6.5.1
Automatic defrosting of the cryochamber ............................................................................................ 30
6.5.2
Manual defrosting of the cryochamber ................................................................................................ 30
6.5.3
Manual defrosting of the quick-freeze shelf ....................................................................................... 31