Leica Biosystems CM1950 - Manual (Page 35)

35 Leica CM1950 – Cryostat
Control panel field 2 – Electric coarse feed, sectioning and trimming thickness display
1
Specimen head forwards – pressing briefly
advances the specimen 20 µm
Specimen head fast
forward
Fig. 37
Move specimen head backwards slowly – pressing
briefly retracts the specimen 20 µm
Move specimen head backwards
quickly to home position (latched)
2
LED for trim-
ming and sec-
tion thickness
display
Illuminates in
yellow when the
specimen is in
retraction.
Toggle TRIM and
SECT (LED active).
Rocking mode – only in manual mode;
in rear area, i.e. approx. 12 - 3 o'clock position of the
handwheel (rock handwheel back and forth a short
distance).
Setting section/trim thickness
Use the - keys on the control panel for
setting; 2nd setting range section thickness:
1 - 100 µm
7. Instrument Controls
Retraction: off = 0
on = 20 µm
In manual mode.
Trimming section thickness setting range: 1 – 600 µm
(Recommended for research applications)
Trimming section thickness setting range:
(Recommended for clinical applications)
Values: 10 µm, 20 µm, 30 µm, 40 µm.
Press approx. 3 sec.;
"on" or "off" appears (for the
retraction). Toggle using the
"+" or "-" key
For motorized sectioning,
the retraction value is fixed
and cannot be changed ( see
Chap. 8, "Control panel field 2" ).
Values
1.0 µm – 10.0 µm in 1.0 µm increments
10.0 µm – 20.0 µm in 2.0 µm increments
20.0 µm – 50.0 µm in 5.0 µm increments
50.0 µm – 100.0 µm in 10.0 µm increments
100.0 µm – 600.0 µm in 50.0 µm increments
Values
1.0 µm – 5.0 µm in 0.5 µm increments
5.0 µm – 20.0 µm in 1.0 µm increments
20.0 µm – 60.0 µm in 5.0 µm increments
60.0 µm – 100.0 µm in 10.0 µm increments
In the "off" setting, there
is no retraction in manu-
al, automatic or rocking
mode.
For trimming values with a
section thickness greater
than 200 µm, the display
flashes to expressly notify
the user of thick sections!