C.B.S. Scientific DH Series - Manual (Page 19)

C.B.S. œ Scientific
19
Dedicated Height Sequencer
4.4 Post-Electrophoresis – Gel Processing
A. Removal of sequencing plate from gel unit.
1. Ensure power supply is OFF disconnect power cords from reservoirs.
2. Reduce buffer level in upper reservoir below level of notch (or drain entirely). If buffer
is not reused, drain buffer from lower reservoir. Be careful with lower reservoir buffer,
it will be radioactive if you have used 32P, 33P or 35S. Dispose of buffer according to
your institutions radioactive safety policies. Unincorporated nucleotides will have run
off the gel.
3. Un-clamp glass plate sandwich from upper reservoir. Wipe gel plate sandwich with a
small piece of paper towel and dispose of contaminated paper in your radioactive
waste receptacle for dry waste.
B. Transfer of gel onto blotting paper
1. Place the sequencing gel on the bench with the short (notched) side up. To
disassemble the gel sandwich remove the gel sealing tape from sides of the
sandwich. Using the C.B.S. Scientific Wedge Plate Separator, insert the wedge about
1/3 of its length between the glass plates, either just inside the "ear" at the top of the
plate, or just inside the spacer along the bottom of the plate. Continue to push with
steady force and the plates will release from the gel. If necessary, slightly twist the
wedge for accelerated results. Do not use corner of Wedge Plate Separator as it will
damage plastic. See illustration below.