C.B.S. Scientific DH Series - Manual (Page 12)

C.B.S. œ Scientific
12
Dedicated Height Sequencer
3.4 Methods for sealing glass plate sandwich
(A) Gel Slider
™, ( B) Capillary Gel Casting or (C) Yellow tape
(A) C.B.S. Scientific's Gel Slider
™, Sequencing Gel Caster. This adjustable width unit
simplifies the pouring of problem-free sequencing gels for the novice or experienced
sequencer. Unit sold separately, see instructions for Gel Slider
™ and skip section 3.5.
(B) Capillary Gel Casting. Depending upon user preference, capillary gel casting can be
performed (1) by using a bottom spacer at the bottom of the gel plate assembly, (2) by
taping the bottom section with yellow tape, or (3) by leaving the end completely open.
Assemble glass plate sandwich with side spacers on a level working surface.
1. If using a bottom spacer, offset the side spacers to accommodate the bottom
spacer, so the spacers line up with the edges of the back plate. Clamp
continuously with casting clamps (cat.# GPC-0002) as shown in illustration.
2. If using tape for the bottom section of the glass plate assembly, extend the tape
around the bottom corners in both directions to ensure an adequate seal. Apply
the tape as smoothly as possible to avoid forming air channels or bubbles along
the edges of the glass plates. Rub the tape firmly onto glass to eliminate air
channels and ensure a liquid-tight seal. Clamp the side sections of the glass plate
assembly with casting clamps (cat.# GPC-0002).
#GPC-0002
3. If leaving the bottom of the glass plate assembly open, clamp the side sections of
the glass plate assembly with casting clamps (cat.# GPC-0002).