C.B.S. Scientific DH Series - Manual (Page 18)

C.B.S. œ Scientific
18
Dedicated Height Sequencer
4.3 Electrophoresis
1. After loading the samples, close the reservoir safety cover. Connect the DC power leads to
the unit then to the power supply with the proper polarity. Make sure the black leads are
connected to the black cathodes (-) and the red leads are connected to the red anode (+).
2. Turn on the power supply and set the voltage or wattage to the proper setting for the gel. Be
sure to follow manufacturer's instructions. (See Table 5.1 for recommended DC power
settings). As a general rule, the applied voltage is approximately 5-6V per cm of gel. Monitor
temperature using the Temperature strip (CBS Cat. # EGT-100).
3. Monitor the progress of electrophoresis by following migration of a marker dye front or by any
other preferred method.
4. When electrophoresis is complete, TURN POWER SUPPLY OFF. Disconnect both DC
power cords, first from the power supply.