C.B.S. Scientific FCU-1000 - Manual (Page 8)

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3.1 Preparation
a. Assemble all of the needed components in the area where you will set up the chamber
(the chamber, the tubing, forceps, pump, respirator, the clamps, and connectors)
b. Prepare chemokines, buffers, etc.
c. If you are going to perform your experiments at 37˚C, pre-warm all the media to avoid
spontaneous bubble formation.
3.2 Setting Up the Tubing
The 3D Flow Chamber Device is intended to be connected in a closed loop for most
experiments - the loop will consist of the chamber, tubing, pump tubing (is a fixed length
and has color coded stops attached) and the respirator.
a. Set up the pump tubing and clip tubing into the pump.
b. Complete the tubing loop
SECTION 3 FLOW CHAMBER SET-UP & USE