C.B.S. Scientific FCU-1000 User Manual
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T A B L E O F C O N T E N T S
Section 1: General Information
1.1 Flow rate chart. . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.2 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3 System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.4 System Contents . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Section 2: Cell Culture on Upper and Lower Inserts
2.1 Coating the Membrane disk . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Growing the Cells . . . . . . . . . . . . . . . . . . . . . . . . .6-7
Section 3: Flow Chamber Set-Up & Use
3.1 Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.2 Setting up the Tubing . . . . . . . . . . . . . . . . . . . . . . . .8-9
3.3 Priming the Tubing . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.4 Filling the Reservoirs and Assembly of 3D Chamber . . . . . . . . . . . 11-13
3.5 Introducing Circulating Cells to Flow Chamber. . . . . . . . . . . . . . . 13
3.6 Recovery of Cells from Chamber . . . . . . . . . . . . . . . . . . .14-16
3.7 Cleaning the Chmaber after Use . . . . . . . . . . . . . . . . . . . . 17
Section 4: Tips and Troubleshooting
4.1 Media Considerations . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2 Pump Controller . . . . . . . . . . . . . . . . . . . . . . . . .19-22
4.2 Flow Rate Chart . . . . . . . . . . . . . . . . . . . . . . . . . . 23