C.B.S. Scientific FCU-1000 - Manual (Page 18)

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4.1. Media Considerations
• All fluids should be equilibrated to temperature to avoid bubble formation.
• Maintenance of pH by CO2 buffering must be achieved by placing the respirator into a Tissue
culture incubator with a loose fitting Petri dish cover on the top of it allowing gas exchange
into the system.
If you would like to bypass the Respirator, an alternative is to use medium that can be buffered
without CO2 such as CO2-independent media (Invitrogen cat# 18045088) which can be sup-
plemented with typical additives such as growth factors and FBS to grow cells. For example,
for HUVEC, CO2-independent media is supplemented with the EGM-2 bullet kit (Lonza)
SECTION 4 ADDITIONAL TIPS AND TROUBLESHOOTING
4.2. Pump Controller