C.B.S. Scientific FCU-1000 - Manual (Page 13)

www.cbsscientific.com 3-D Flow Chamber Device Instructions April 2014 13
d. Start the pump (direction towards the respirator) - 1-2 mls/min. Tilt the chamber slightly upwards to facili-
tate bubble clearance from the chamber. You also may tap it lightly to dislodge any bubbles. Once buffer
is coming out the other side of the chamber attach the tubing to close the loop.
e. Reduce the pump speed to desired level for experiment (see appendix).
3.5. Introducing Circulating Cells to the Flow Chamber without Disrupting the Flow.
a. Prepare cells to desired density. 1-10million/ml is a common range although varying levels may need to
be tested to ensure compatibility with the application.
b. With the pump running, dispense the cells into the respirator as shown.
c. If desired, place entire apparatus including the pump into a tissue culture incubator.