C.B.S. Scientific FCU-1000 User Manual
Page 7
www.cbsscientific.com
3-D Flow Chamber Device Instructions April 2014
7
a. Remove any liquid remaining from the coating and dispense the cells onto the membrane as shown above. The
liquid will bead up as it did when coating.
b. Place the covered petri dish in the incubator and incubate for the desired amount time – most cells will attach
within 1 hour.
c. Fill the dish with medium so that the membranes are floating. The top membrane is prone to filling up
with air – if this happens you can pick the membrane up with forceps and pipet some of the media
into the underside of the membrane and then slowly lay it down onto the media so that it is wet from
below.
d. Place the dish in the incubator. The cells can be stained with a live cell dye to facilitate viewing them
on the membranes (example: calcein AM or cell tracker dyes).
e. Observe cells and plan to use immediately when they reach the desired density. Confluent HUVEC
cells stained with calcein AM are shown below.