C.B.S. Scientific FCU-1000 User Manual
Page 6
6
2.1. Coat the membrane disk with the relevant substrate to support cell adhesion
a. Using aseptic technique (sterile forceps) place the dry membrane disk on a dry sterile petri dish
The top membrane is positioned so that the membrane support (clear ring) is on the bottom and the
membrane is facing up.
The bottom membrane is positioned so that the membrane support is facing up – the membrane disk
will look like a little cup.
b. Carefully dispense the coating of interest (example: fibronectin, poly-l-lysine) onto the membrane
surface. The membrane is hydrophobic, be sure that the liquid covers the entire surface. It will
bead up. It will take approximately 500μl to cover the top membrane and the same amount will fill
the bottom membrane cup.
c. Cover up the petri dish and incubate at the desired temperature for the desired duration.
d. Wash the coating as necessary. The membrane is now ready for use with cells.
2.2 Growing cells on the coated membrane
SECTION 2 CELL CULTURE ON UPPER & LOWER INSERTS