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C.B.S. Scientific FCU-1000 User Manual

Page 10

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3.3 Priming the Tubing in Preparation for an Experiment.

a. Prepare a 50 ml tube with your buffer of interest place the tubing (right side, not the Respirator side) into

the tube. Run the pump so that it is pumping in the direction of the Respirator. A flow rate of ~2ml/min is a

good starting point. Make sure the pinch clamp is closed on the Respirator side.

b. Fill the Respirator, cover it and release the pinch clamp when ready to connect to 3D chamber.