C.B.S. Scientific FCU-1000 - Manual (Page 11)

www.cbsscientific.com 3-D Flow Chamber Device Instructions April 2014 11
3.4. Filling the Reservoirs and Assembling the 3D Chamber.
a. If you are using 2 membranes as a sandwich fill the reservoir with ~800 ul of chemokine or buffer. If you are using
the single top membrane fill the chamber with ~1.5ml of chemokine or buffer.
b. Work quickly! If using the double membrane, first pop the membranes together and then lay them into the reservoir.
If using a single membrane, lay it over the buffer using forceps. In either case, tamp the membranes down flush with
the top of the chamber and remove excess buffer if it overflows from the reservoir using a pipet.