Eppendorf Reference - Manual (Page 20)

19
Error Cause Solution
Droplets on
the inner wall
of the pipette
tip. – Uneven wetting of the
plastic wall.
– A pipette tip with poor
wetting properties has
been used.
– Attach a new pipette tip.
– Use an original eppendorf
tip.
Pipette is
dripping
and/or the
volume
pipetted is
incorrect. – The tip is loose.
– A poorly fitting pipette tip
has been used.
– Liquid with a high vapor
pressure has been pipetted.
– Tip has been taken out of
the liquid too quickly.
The pipette is dripping
because:
– Piston is contaminated.
– Piston is damaged.
– Seals are damaged.
– Tip cone loose.
– Press the tip firmly in place.
– Use an original eppendorf
tip.
– In this case, we recommend
pipetting using a positive-
displacement pipette.
– Remove the tip slowly from
the liquid.
– Clean and lightly lubricate
the piston.
– Replace the piston and seal
(see Part B, Maintenance).
– Replace all seals
(see Part B, Maintenance).
– Lightly tighten tip cone with
wrench (see Part B,
Maintenance).
Exchange, where
necessary.
10 Troubleshooting
Eppendorf Reference® – Part A – Chapter 10