Eppendorf Reference - Manual (Page 17)

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d Pierce the calibration seal attached to the opening
for adjustments with Side B of the key provided.
This destroys the seal which should be removed.
Carefully attach the key to the adjusting sleeve
inside.
e Turn the wrench to adjust the volume display of the pipette (with piston stroke
unchanged) to the actual volume (measurement under step c).
f Remove the wrench.
g Repeat step c). The readings must be within the tolerances specified in the
technical data.
If the nominal value still does not agree with the measuring result, repeat
steps d – g.
Since this adjustment affects the entire measuring range, it is imperative to check
the other volumes of this pipette specified in the technical data.
Then reseal the adjustment opening using one of the calibration seals supplied.
8.2.3 Adjustment for liquids with a density different to that of water
It is possible to adjust the pipette for one specific volume of liquid with a density
different to that of water in such a way that the volume displayed corresponds to
the volume pipetted.
All other values for the adjustable pipettes are now out of alignment, i.e. an
adjustable-volume pipette has been converted into a fixed-volume pipette!
Proceed as described in Chapter 8.2.2.
A pipette set in this way delivers a pipetting value that correlates with that in
the display only for the liquid used and for the volume tested! For this
reason, we very strongly recommend labeling the converted pipette as a
fixed-volume pipette for "Solution y”!
The error for liquids with a higher vapor pressure (e.g. organic solvents)
cannot be aligned in this way. In this case, we recommend using an
eppendorf positive-displacement pipette.
C A L
Eppendorf Reference® – Part A – Chapter 8